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KMID : 1161520060100010015
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Animal Cells and Systems
2006 Volume.10 No. 1 p.15 ~ p.20
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Cytokines regulate the expression of the thymus and activation-regulated chemokine (TARC; CCL17) in human skin fibroblast cells
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Lee Ji-Sook
Kim In-Sik
Kim Dong-Hee
Yun Chi-Young
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Abstract
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Allergic inflammation is thought to be a Th2 cell?dominant immune response during which tissue?resident fibroblasts produce chemokines which contribute to the recruitment of migratory leukocytes to sites of tissue injury. Thymus and activation?regulated chemokine (TARC; CCL17) is a potent member of the CC chemokine family and a selective chemoattractant for Th2 cells. In order to study the regulatory profiles of TARC production by TNF?α, IFN?γ, and IL?4 in human normal skin fibroblast, CCD?986sk cell line was used. The expression of TARC protein was measured using ELISA, and mRNA level was detected by RT?PCR. The combination of TNF?α and IL?4 induced a time? and dose?dependent synergistic increase in the expression of TARC at both protein and mRNA levels in the cultured human skin fibroblasts. Exposure of the cells to single cytokine had no effect on TARC expression. The high concentration (100 ng/ml) and long incubation time (72 h) of IFN?γ further enhanced the TARC production induced by TNF?α/IL?4 in the skin fibroblast. This synergistic effect of Th1 and Th2 type cytokines on TARC production by skin fibroblasts may contribute to the inflammatory cell infiltration and tissue damage with allergic inflammation.
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KEYWORD
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Allergic inflammation, skin fibroblast cell, thymus and activation-regulated chemokine (TARC, CCL17), CCD986sk cell
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